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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- AM09008PU-N - Provider product page
- Provider
- OriGene
- Product name
- Eph receptor A2 (EPHA2) mouse monoclonal antibody, clone 3D7, Purified
- Antibody type
- Monoclonal
- Description
- Eph receptor A2 (EPHA2) mouse monoclonal antibody, clone 3D7, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- EPHA2
- Isotype
- IgG
- Antibody clone number
- 3D7
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis on mouse brain: The extracts of mouse brain (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EphA2 antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: Paraffin embedded sections of human colon and gastric cancer tissue were incubated with anti-human EphA2 antibody (1/100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: Formalin-Fixed, Paraffin-Embedded Human Thyroid stained with EPHA2 antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: Formalin-Fixed, Paraffin-Embedded Human Testis stained with EPHA2 antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.
- Validation comment
- IHC
