Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C755452 - Provider product page
- Provider
- LSBio
- Product name
- EPHA2 / EPH Receptor A2 Antibody (clone AT66G9) LS-C755452
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AT66G9
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : PC3 cell lysate Recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHA2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human EPHA2 extracellular domain Lane 2 : Recombinant human EPHA2 cytoplasmic domain