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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP14274PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP14274PU-N, RRID:AB_1751970
- Product name
- anti EPHA2 / ECK (N-term)
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38~67 amino acids from the N-terminal region of Human EphA2.
- Reactivity
- Human, Mouse, Hamster
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- LEFT: Western blot analysis of hEPHA2-T45 in MCF-7 cell line lysates (35ug/lane). EPHA2 (arrow) was detected using the purified Pab. MIDDLE: Western blot analysis of hEPHA2-T45 in mouse NIH-3T3 cell line lysates (35ug/lane). EPHA2 (arrow) was detected using the purified Pab. RIGHT: Western blot analysis of EPHA2 (arrow) using rabbit polyclonal hEPHA2-T45. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of NCI-H292 cells using EphA2 Antibody (N-term) (AP14274PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
