- GTX102993 - GeneTex CDK4 antibody

Submitted references Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.

Liu YH, Weng YP, Tsai HY, Chen CJ, Lee DY, Hsieh CL, Wu YC, Lin JY
Phytomedicine : international journal of phytotherapy and phytopharmacology 2018 Jul 15;46:184-192

Regulation of miR-34b/c-targeted gene expression program by SUMOylation.

Li YJ, Du L, Aldana-Masangkay G, Wang X, Urak R, Forman SJ, Rosen ST, Chen Y
Nucleic acids research 2018 Aug 21;46(14):7108-7123

Licochalcone A attenuates glioma cell growth in vitro and in vivo through cell cycle arrest.

Lu WJ, Wu GJ, Chen RJ, Chang CC, Lien LM, Chiu CC, Tseng MF, Huang LT, Lin KH
Food & function 2018 Aug 15;9(8):4500-4507

Cell-Cycle Proteins Control Production of Neutrophil Extracellular Traps.

Amulic B, Knackstedt SL, Abu Abed U, Deigendesch N, Harbort CJ, Caffrey BE, Brinkmann V, Heppner FL, Hinds PW, Zychlinsky A
Developmental cell 2017 Nov 20;43(4):449-462.e5

Miconazole induces apoptosis via the death receptor 5-dependent and mitochondrial-mediated pathways in human bladder cancer cells.

Yuan SY, Shiau MY, Ou YC, Huang YC, Chen CC, Cheng CL, Chiu KY, Wang SS, Tsai KJ
Oncology reports 2017 Jun;37(6):3606-3616

Serine 235 Is the Primary NS5A Hyperphosphorylation Site Responsible for Hepatitis C Virus Replication.

Hsu SC, Lo CW, Pan TC, Lee KY, Yu MJ
Journal of virology 2017 Jul 15;91(14)

Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.

Liu YH, Weng YP, Lin HY, Tang SW, Chen CJ, Liang CJ, Ku CY, Lin JY
Scientific reports 2017 Jan 30;7:41437

Fibroblast growth factor 9 (FGF9) regulation of cyclin D1 and cyclin-dependent kinase-4 in ovarian granulosa and theca cells of cattle.

Totty ML, Morrell BC, Spicer LJ
Molecular and cellular endocrinology 2017 Jan 15;440:25-33

Hinokitiol Negatively Regulates Immune Responses through Cell Cycle Arrest in Concanavalin A-Activated Lymphocytes.

Chung CL, Leung KW, Lu WJ, Yen TL, He CF, Sheu JR, Lin KH, Lien LM
Evidence-based complementary and alternative medicine : eCAM 2015;2015:595824

Methoxyacetic acid suppresses prostate cancer cell growth by inducing growth arrest and apoptosis.

Parajuli KR, Zhang Q, Liu S, Patel NK, Lu H, Zeng SX, Wang G, Zhang C, You Z
American journal of clinical and experimental urology 2014;2(4):300-12

Combination therapy targeting ectopic ATP synthase and 26S proteasome induces ER stress in breast cancer cells.

Chang HY, Huang TC, Chen NN, Huang HC, Juan HF
Cell death & disease 2014 Nov 27;5:e1540

Cephalochromin induces G0/G1 cell cycle arrest and apoptosis in A549 human non-small-cell lung cancer cells by inflicting mitochondrial disruption.

Hsiao CJ, Hsiao G, Chen WL, Wang SW, Chiang CP, Liu LY, Guh JH, Lee TH, Chung CL
Journal of natural products 2014 Apr 25;77(4):758-65

UBE2M-mediated p27(Kip1) degradation in gemcitabine cytotoxicity.

Huang AM, Kao YT, Toh S, Lin PY, Chou CH, Hu HT, Lu CY, Liou JY, Chao SY, Hour TC, Pu YS
Biochemical pharmacology 2011 Jul 1;82(1):35-42

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (GTX102993) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: <50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 39883

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample(30 ?g of whole cell lysate)A:293T 12% SDS PAGEGTX102993 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A:NIH-3T312% SDS PAGE GTX102993 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (GTX102993) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (GTX102993) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (GTX102993) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK4 antibody (GTX102993) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: CDK4 antibody detects CDK4 protein at nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:1000.Red: p21 Cip1, a nucleus marker, stained by p21 Cip1 antibody [GT1032] (GTX629543) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: CDK4 antibody detects CDK4 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody (GTX102993) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CDK4(GTX102993) antibody at 1:500 dilution.

GTX102993

Antibody data