H00001019-M03

antibody from Abnova Corporation

Targeting: CDK4 PSK-J3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001019-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001019-M03, RRID:AB_565575

Product name

CDK4 monoclonal antibody (M03), clone 4F11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant CDK4.

Antigen sequence

KPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLP
RGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNP
HKRISAFRALQHSYLHKDEGNPE


Isotype

IgG

Antibody clone number

4F11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11.Lane 1: CDK4 transfected lysate(33.7 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CDK4 monoclonal antibody (M03), clone 4F11. Western Blot analysis of CDK4 expression in Hela S3 NE.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CDK4 monoclonal antibody (M03), clone 4F11. Western Blot analysis of CDK4 expression in 293.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CDK4 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol