Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00001019-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001019-M03, RRID:AB_828716
- Product name
- Mouse Monoclonal CDK4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CDK4 (4F11)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Western blot analysis of CDK4 over-expressed 293 cell line. Lane 2 cotransfected with CDK4 Validated Chimera RNAi or non-transfected control. Blot probed with H00001019-M03. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11. Lane 1: CDK4 transfected lysatE (33.7 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in Hela S3 NE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of CDK4 expression in 293.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CDK4 Antibody (4F11) [H00001019-M03] - Detection limit for recombinant GST tagged CDK4 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: CDK4 Antibody (4F11) [H00001019-M03] - Analysis of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration 10 ug/ml