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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB2914 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2914, RRID:AB_1573895
- Product name
- CDK4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CDK4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cyclins and cyclin-dependent kinases: comparative study of hepatocellular carcinoma versus cirrhosis.
Phosphorylation of p16INK4A correlates with Cdk4 association.
Absence of R24C mutation of the CDK4 gene in leukemias and solid tumors.
Masaki T, Shiratori Y, Rengifo W, Igarashi K, Yamagata M, Kurokohchi K, Uchida N, Miyauchi Y, Yoshiji H, Watanabe S, Omata M, Kuriyama S
Hepatology (Baltimore, Md.) 2003 Mar;37(3):534-43
Hepatology (Baltimore, Md.) 2003 Mar;37(3):534-43
Phosphorylation of p16INK4A correlates with Cdk4 association.
Gump J, Stokoe D, McCormick F
The Journal of biological chemistry 2003 Feb 28;278(9):6619-22
The Journal of biological chemistry 2003 Feb 28;278(9):6619-22
Absence of R24C mutation of the CDK4 gene in leukemias and solid tumors.
Mori N, Yang R, Kawamata N, Miller CW, Mizoguchi H, Koeffler HP
International journal of hematology 2003 Apr;77(3):259-62
International journal of hematology 2003 Apr;77(3):259-62
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CDK4 polyclonal antibody (Cat # PAB2914) in HL-60 cell lysate. CDK4 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDK4 polyclonal antibody (Cat # PAB2914) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HL-60 cells using CDK4 polyclonal antibody (Cat # PAB2914)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
