PA5-59088

antibody from Invitrogen Antibodies

Targeting: TGM1 ICR2, LI, LI1, TGASE, TGK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-59088

Provider

Invitrogen Antibodies

Product name

TGM1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MMDGPRSDVG RWGGNPLQPP TTPSPEPEPE PDGRSRRGGG RSFWARCCGC CSCRNAADDD WGPEPSDSRG RGSSSGTRRP GSRGSDSRRP VSRGSGVNAA Highest antigen sequence identity to the following orthologs: Mouse - 74%, Rat - 74%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Trichohyalin-like 1 protein plays a crucial role in proliferation and anti-apoptosis of normal human keratinocytes and squamous cell carcinoma cells.

Makino T, Mizawa M, Yoshihisa Y, Yamamoto S, Tabuchi Y, Miyai M, Hibino T, Sasahara M, Shimizu T
Cell death discovery 2020;6:109

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TGM1 in human tonsil tissue using a TGM1 Polyclonal Antibody (Product # PA5-59088).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TGM1 in human esophagus and colon tissues using TGM1 Polyclonal Antibody (Product # PA5-59088). Corresponding TGM1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TGM1 in human esophagus using TGM1 Polyclonal Antibody (Product # PA5-59088) shows moderate to strong membranous positivity in squamous epithelial cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of TGM1 in human colon using TGM1 Polyclonal Antibody (Product # PA5-59088) shows no positivity in glandular cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 TCHHL1 depletion inhibits proliferation in human epidermal tissue. a Skin-equivalent models were prepared using NHKs transfected with TCHHL1 siRNA or control siRNA. A Hematoxylin-Eosin-stained image is shown. Scale bar: 50 mum. b Skin equivalent models treated TCHHL1 siRNA or control siRNA were stained with anti-Ki67 antibody. The arrowheads indicate Ki67-positive cells. Scale bar: 100 mum. c A quantitative analysis of the Ki67-positive cells in the basal layer. Ki67-positive cells were counted in each 500-mum field (total length 5.0 mm) and expressed as the mean +- SD of three independent experiments. *p < 0.01. d Skin-equivalent models treated with TCHHL1 siRNA or control siRNA were stained with antibodies against filaggrin (FLG), transglutaminase 1 (TGM1), keratin 10 (K10), and keratin 14 (K14). The dotted line indicates the dermo-epidermal junction. Scale bar: 50 mum.