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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 11539-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAGED1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: PIPPDWQNL RPSPNLRPSP NSRASQNPGA AQPRDVALLQ ERANKLVKYL MLKDYTKVPI KRSEMLRDII REYTDVYPEI IERACFVLEK KFGIQLKEID KEEHLYILIS TPESLAGILG TTKDTPKLGL LLVILGVIFM NGNRASEAVL WEALRKMGLR PGVRHPLLGD LRKLLTYEFV KQKYLDYRRV PNSNPPEYEF LWGLRSYHET SKMKVLRFIA EVQKRDPRDW TAQFMEAADE ALDALDAAAA EAEARAEART RMGIGDEAVS GPWSWDDIEF ELLTWDEEGD FGDPWSRIPF TFWARYHQNA RSRFPQTFAG PIIGPGGTAS ANFAANFGAI GFFWVE (490-834 aa encoded by BC014070)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 11539-1-AP (MAGED1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11539-1-AP ( MAGED1 Antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11539-1-AP ( MAGED1 Antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug MAGED1 antibody (11539-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
