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Learn22053-1-AP
antibody from Invitrogen Antibodies
Targeting: MAGED1
DLXIN-1, NRAGE
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 22053-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAGED1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAQKMDCGA GLLGFQNPDA CRAVCHPLPQ PPASTLPLSA FPTLCDPPYS QLRDPPAVLS CYCTPLGASP APAEASVEDS ALLMQTLMEA IQISEAPPTN QATAAASPQS SQPPTANEMA DIQVSAAAAR PKSAFKVQNA TTKGPNGVYD FSQAHNAKDV PNTQPKAAFK SQNATPKGPN AAYDFSQAAT TGELAANKSE MAFKAQNATT KVGPNATYNF SQSLNANDLA NSRPKTPFKA WNDTTKAPTA DTQTQNVNQA KMATSQADIE TDPGISEPDG ATAQTSADGS QAQNLESRTI IRGKRTRKIN NLNVEENSSG DQRRAPLAAG TWRSAPVPVT TQNPPGA (1-346 aa encoded by BC014070)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.34 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse placenta tissue were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP ( MAGED1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 22053-1-AP (MAGED1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A549 cells using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 22053-1-AP (MAGED1 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with .2ug MAGED1 antibody (22053-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-MAGED1 (IP:22053-1-AP, 4ug; Detection:22053-1-AP 1:1000) with A549 cells lysate 400ug.
