PA5-65820

antibody from Invitrogen Antibodies

Targeting: XPO4 FLJ13046, KIAA1721

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-65820

Provider

Invitrogen Antibodies

Product name

XPO4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: DIHWLILVTGY LLADDTQGET PLIPPEIMEY SIKHSSEVDI NTTLQILGSP GEKASSIPGY NRTDSVIRLL SAILRVSEVE SRAIRADLTH LLSPQMG

Concentration

0.1 mg/mL

References

Submitted references

Unconventional tonicity-regulated nuclear trafficking of NFAT5 mediated by KPNB1, XPOT and RUVBL2.

Cheung CY, Huang TT, Chow N, Zhang S, Zhao Y, Chau MP, Chan WC, Wong CCL, Boassa D, Phan S, Ellisman MH, Yates JR, Xu S, Yu Z, Zhang Y, Zhang R, Ng LL, Ko BCB
Journal of cell science 2022 Jul 1;135(13)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of XPO4 in human cell line U-2 OS shows localization to nucleoplasm and cytosol. Samples were probed using a XPO4 Polyclonal Antibody (Product # PA5-65820).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4. Identification of importin and exportin for NFAT5 nuclear import and export. (A) In vitro nuclear import assay of His-SV40 NLS -AcGFP and His-NFAT5 171-250 -AcGFP was carried out using digitonin-permeabilized HeLa cells supplemented with individual nuclear transport factors, as indicated. The transport assay was carried out for 30 min at 37degC. Cells were then fixed with paraformaldehyde and stained with DAPI, and GFP fluorescence images were taken using a confocal microscope. Scale bar: 30 um. Images are representative of three independent experiments. (B) Role of KPNB1 in nuclear import of His-NFAT5-AcGFP. Left: schematic representation of the indicated His-NFAT5-AcGFP reporter constructs. Shaded regions indicate NFAT5-cNLS. Right: in vitro nuclear import assay of His-SV40 NLS -AcGFP and the indicated His-NFAT5-AcGFP fusions was carried out using digitonin-permeabilized HeLa cells supplemented with RanGTP, NTF2 and ATP-regenerating mixture, in the presence or absence of KPNB1. The transport assay was carried out for 30 min at 37degC. Cells were then fixed with paraformaldehyde and stained with DAPI, and images were taken using a confocal microscope. Scale bar: 30 um. Images are representative of three independent experiments. (C) Quantitative analysis of subcellular localization of immunofluorescence signal in HeLa cells transfected with FLAG-NFAT5 132-581 and siRNA targeting the indicated members of the exportin family. Cells were switched to isotonic (Iso) or hyp