17037-1-AP
antibody from Invitrogen Antibodies
Targeting: CHAF1A
CAF-1, CAF1, CAF1B, CAF1P150, MGC71229, P150
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 17037-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHAF1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: EEPGESLSH SEGDDDDDMG EDEDEDDGFF VPHGYLSEDE GVTEECADPE NHKVRQKLKA KEWDEFLAKG KRFRVLQPVK IGCVWAADRD CAGDDLKVLQ QFAACFLETL PAQEEQTPKA SKRERRDEQI LAQLLPLLHG NVNGSKVIIR EFQEHCRRGL LSNHTGSPRS PSTTYLHTPT PSEDAAIPSK SRLKRLISEN SVYEKRPDFR MCWYVHPQVL QSFQQEHLPV PCQWSYVTSV PSAPKEDSGS VPSTGPSQGT PISLKRKSAG SMCITQFMKK RRHDGQIGAE DMDGFQADTE EEEEEEGDCM IVDVPDAAEV QAPCGAASGA GGGVGVDTGK ATLTASPLGA S (607-956 aa encoded by BC067093)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17037-1-AP (CHAF1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 17037-1-AP (CHAF1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervix tissue slide using 17037-1-AP ( CHAF1A Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervix tissue slide using 17037-1-AP (CHAF1A Antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CHAF1A (IP:17037-1-AP, 4ug; Detection:17037-1-AP 1:1000) with Jurkat cells lysate 2400ug.