Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB8700 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB8700, RRID:AB_10715122
- Product name
- LDHA monoclonal antibody, clone AT1A4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant LDHA.
- Isotype
- IgG
- Antibody clone number
- AT1A4
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Inhibition of lactate dehydrogenase A induces oxidative stress and inhibits tumor progression.
LDH-A inhibition, a therapeutic strategy for treatment of hereditary leiomyomatosis and renal cell cancer.
The ldhA gene, encoding fermentative L-lactate dehydrogenase of Corynebacterium glutamicum, is under the control of positive feedback regulation mediated by LldR.
Le A, Cooper CR, Gouw AM, Dinavahi R, Maitra A, Deck LM, Royer RE, Vander Jagt DL, Semenza GL, Dang CV
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 2;107(5):2037-42
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 2;107(5):2037-42
LDH-A inhibition, a therapeutic strategy for treatment of hereditary leiomyomatosis and renal cell cancer.
Xie H, Valera VA, Merino MJ, Amato AM, Signoretti S, Linehan WM, Sukhatme VP, Seth P
Molecular cancer therapeutics 2009 Mar;8(3):626-35
Molecular cancer therapeutics 2009 Mar;8(3):626-35
The ldhA gene, encoding fermentative L-lactate dehydrogenase of Corynebacterium glutamicum, is under the control of positive feedback regulation mediated by LldR.
Toyoda K, Teramoto H, Inui M, Yukawa H
Journal of bacteriology 2009 Jul;191(13):4251-8
Journal of bacteriology 2009 Jul;191(13):4251-8
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis from cell lysate of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with LDHA monoclonal antibody, clone AT1A4 (Cat # MAB8700) (1 : 8000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and LDHA monoclonal antibody, clone AT1A4 (Cat # MAB8700) (1 : 500) with Texas Red (Red).
- Validation comment
- Immunofluorescence
