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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- TA807653 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA807653, RRID:AB_2628542
- Product name
- LIME1 mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)
- Antibody type
- Monoclonal
- Description
- LIME1 mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LIME1
- Isotype
- IgG
- Antibody clone number
- OTI3B10
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIME1 (RC201909, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIME1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LIME1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807653)
- Validation comment
- IHC
