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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- SM3137P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM3137P, RRID:AB_1004599
- Product name
- anti LIME1
- Antibody type
- Monoclonal
- Antigen
- Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- LIME-06
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with anti-human CD3 (MEM-92; SM3152P) or anti-human CD4 (MEM-16; AM03098PU-N), and LIME was immunoprecipitated from laurylmaltoside lysates with the LIME-06 antibody (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).
