Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- UM800097 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800097, RRID:AB_2629206
- Product name
- Troponin T (TNNT2) mouse monoclonal antibody,clone UMAB205
- Antibody type
- Monoclonal
- Description
- Troponin T (TNNT2) mouse monoclonal antibody,clone UMAB205
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TNNT2
- Isotype
- IgG
- Antibody clone number
- UMAB205
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (25ug) from 4 different cell lines by using anti-TNNT2 monoclonal antibody. (1:500) (1: HEK293; 2: Hela; 3: MCF7; 4: HepG2) Dilution: 1:500
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot of human tissue lysates (15ug) from 2 different tissues (1: Heart, 2: Muscle). Diluation: 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-TNNT2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800097)(1:200)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TNNT2 mouse monoclonal antibody (UM800097). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
- Validation comment
- 10K-CHIP