MA5-13308

antibody from Invitrogen Antibodies

Targeting: CD5 LEU1, T1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-13308

Provider

Invitrogen Antibodies

Product name

CD5 Monoclonal Antibody (4C7)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-13308 targets CD5 in IHC (P), FACS and WB applications and shows reactivity with Human samples.

Antibody clone number

4C7

Concentration

Conc. Not Determined

References

Submitted references

Utility of a Reverse Phase Protein Array to Evaluate Multiple Biomarkers in Diffuse Large B-Cell Lymphoma.

Suzuki M, Muroi A, Nojima M, Numata A, Takasaki H, Sakai R, Yokose T, Miyagi Y, Koshikawa N
Proteomics. Clinical applications 2020 Jan;14(1):e1900091

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Cytomorphology and flow cytometry of brain biopsy rinse fluid enables faster and multidisciplinary diagnosis of large B-cell lymphoma of the central nervous system.

Debliquis A, Voirin J, Harzallah I, Maurer M, Lerintiu F, Drénou B, Ahle G
Cytometry. Part B, Clinical cytometry 2018 Jan;94(1):182-188

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Clonal evolution in patients with chronic lymphocytic leukaemia developing resistance to BTK inhibition.

Burger JA, Landau DA, Taylor-Weiner A, Bozic I, Zhang H, Sarosiek K, Wang L, Stewart C, Fan J, Hoellenriegel J, Sivina M, Dubuc AM, Fraser C, Han Y, Li S, Livak KJ, Zou L, Wan Y, Konoplev S, Sougnez C, Brown JR, Abruzzo LV, Carter SL, Keating MJ, Davids MS, Wierda WG, Cibulskis K, Zenz T, Werner L, Dal Cin P, Kharchencko P, Neuberg D, Kantarjian H, Lander E, Gabriel S, O'Brien S, Letai A, Weitz DA, Nowak MA, Getz G, Wu CJ
Nature communications 2016 May 20;7:11589

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Infectious mononucleosis mimicking malignant T-cell lymphoma in the nasopharynx: a case report and review of the literature.

He HL, Wang MC, Huang WT
International journal of clinical and experimental pathology 2013;6(1):105-9

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Clinicopathology, immunophenotype, T cell receptor gene rearrangement, Epstein-Barr virus status and p53 gene mutation of cutaneous extranodal NK/T-cell lymphoma, nasal-type.

Wang TT, Xu C, Liu SL, Kan B, Ran YP, Liu WP, Li GD, Wang L
Chinese medical journal 2013 Apr;126(7):1281-7

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R-CHOP regimen can significantly decrease the risk of disease relapse and progression in patients with non-germinal center B-cell subtype diffuse large B-cell lymphoma.

He XH, Li B, Yang S, Lu N, Zhang X, Zou SM, Li YX, Song YW, Zheng S, Dong M, Zhou SY, Yang JL, Liu P, Zhang CG, Qin Y, Feng FY, Shi YK
Chinese journal of cancer 2012 Jun;31(6):306-14

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Primary follicular lymphoma of the epididymis positive for t(14;18)(q32;q21)/IGH-BCL2 and negative for BCL2 protein expression: a case report.

Tralongo V, Becchina G, Nagar C, Ottoveggio G, Mason S, Seno B, Sessa F, Frattini M, Genovese F
Journal of medical case reports 2012 Jan 18;6:24

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Anaplastic large cell lymphoma with paraneoplastic leukocytosis: a clinicopathological analysis of five cases.

Chang IW, Chen HK, Ma MC, Huang WT
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2011 Nov;119(11):794-801

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Chronic lymphocytic leukemia with t(14;19)(q32;q13) is characterized by atypical morphologic and immunophenotypic features and distinctive genetic features.

Huh YO, Schweighofer CD, Ketterling RP, Knudson RA, Vega F, Kim JE, Luthra R, Keating MJ, Medeiros LJ, Abruzzo LV
American journal of clinical pathology 2011 May;135(5):686-96

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Composite biclonal marginal zone lymphoma of lung and chronic lymphocytic leukemia: pathologic, phenotypic, cytogenetic, and molecular study.

Bhagavathi S, Micale MA, Douglas-Nikitin V, Ballouz S, Neumann K, Blenc AM
International journal of surgical pathology 2011 Jun;19(3):377-81

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Tumour eosinophilia combined with an immunohistochemistry panel is useful in the differentiation of type B3 thymoma from thymic carcinoma.

Khoury T, Chandrasekhar R, Wilding G, Tan D, Cheney RT
International journal of experimental pathology 2011 Apr;92(2):87-96

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Immunohistochemical profile and fluorescence in situ hybridization analysis of diffuse large B-cell lymphoma in northern China.

Li T, Medeiros LJ, Lin P, Yin H, Littlejohn M, Im W, Lennon PA, Hu P, Jorgensen JL, Liang M, Guo H, Yin CC
Archives of pathology & laboratory medicine 2010 May;134(5):759-65

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Clonal relationship of extranodal marginal zone lymphomas of mucosa-associated lymphoid tissue involving different sites.

Konoplev S, Lin P, Qiu X, Medeiros LJ, Yin CC
American journal of clinical pathology 2010 Jul;134(1):112-8

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Intravascular lymphocytosis in acute appendicitis: potential mimicry of chronic lymphocytic leukaemia.

Lee S, Ogilvie RT, Dupre M, Gao ZH
Histopathology 2009 Dec;55(6):660-4

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MYC translocation in chronic lymphocytic leukaemia is associated with increased prolymphocytes and a poor prognosis.

Huh YO, Lin KI, Vega F, Schlette E, Yin CC, Keating MJ, Luthra R, Medeiros LJ, Abruzzo LV
British journal of haematology 2008 Jul;142(1):36-44

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Lymph node involvement by Langerhans cell histiocytosis: a clinicopathologic and immunohistochemical study of 20 cases.

Edelweiss M, Medeiros LJ, Suster S, Moran CA
Human pathology 2007 Oct;38(10):1463-9

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Sequence analysis proves clonal identity in five patients with typical and blastoid mantle cell lymphoma.

Yin CC, Medeiros LJ, Cromwell CC, Mehta AP, Lin P, Luthra R, Abruzzo LV
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2007 Jan;20(1):1-7

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Differential Noxa/Mcl-1 balance in peripheral versus lymph node chronic lymphocytic leukemia cells correlates with survival capacity.

Smit LA, Hallaert DY, Spijker R, de Goeij B, Jaspers A, Kater AP, van Oers MH, van Noesel CJ, Eldering E
Blood 2007 Feb 15;109(4):1660-8

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ZAP70 expression assessed by immunohistochemistry on peripheral blood: a simple prognostic assay for patients with chronic lymphocytic leukemia.

Roullet M, Sargent R, Pasha T, Cajiao I, Elstrom R, Smith T, Liebhaber S, Zhang P, Bagg A
Applied immunohistochemistry & molecular morphology : AIMM 2007 Dec;15(4):471-6

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Lymphomas involving the pleura: a clinicopathologic study of 34 cases diagnosed by pleural biopsy.

Vega F, Padula A, Valbuena JR, Stancu M, Jones D, Medeiros LJ
Archives of pathology & laboratory medicine 2006 Oct;130(10):1497-502

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EBV-associated B- and T-cell posttransplant lymphoproliferative disorders following primary EBV infection in a kidney transplant recipient.

Yin CC, Medeiros LJ, Abruzzo LV, Jones D, Farhood AI, Thomazy VA
American journal of clinical pathology 2005 Feb;123(2):222-8

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Diagnosis and prognosis of B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL) and mantle cell lymphoma (MCL).

Asaad NY, Abd El-Wahed MM, Dawoud MM
Journal of the Egyptian National Cancer Institute 2005 Dec;17(4):279-90

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Inflammatory pseudotumor of the spleen with EBV positivity: report of a case.

Oz Puyan F, Bilgi S, Unlu E, Yalcin O, Altaner S, Demir M, Cakir B
European journal of haematology 2004 Apr;72(4):285-91

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Waldenstrom macroglobulinemia involving extramedullary sites: morphologic and immunophenotypic findings in 44 patients.

Lin P, Bueso-Ramos C, Wilson CS, Mansoor A, Medeiros LJ
The American journal of surgical pathology 2003 Aug;27(8):1104-13

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Epstein-Barr virus-associated lymphoproliferative disease occurring in a patient with sarcoidosis treated by methotrexate and methylprednisolone.

Theate I, Michaux L, Dardenne S, Guiot Y, Brière J, Emile FJ, Fabiani B, Detry R, Gaulard P, Groupe d'Etude des Lymphomes de l'Adulte (GELA)
European journal of haematology 2002 Oct;69(4):248-53

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Epstein-Barr virus-associated lymphoproliferative disease occurring in a patient with sarcoidosis treated by methotrexate and methylprednisolone.

Theate I, Michaux L, Dardenne S, Guiot Y, Brière J, Emile FJ, Fabiani B, Detry R, Gaulard P, Groupe d'Etude des Lymphomes de l'Adulte (GELA).
European journal of haematology 2002 Oct;69(4):248-53

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Epstein-Barr virus-positive B-cell lymphoproliferative disorders arising in immunodeficient patients previously treated with fludarabine for low-grade B-cell neoplasms.

Abruzzo LV, Rosales CM, Medeiros LJ, Vega F, Luthra R, Manning JT, Keating MJ, Jones D
The American journal of surgical pathology 2002 May;26(5):630-6

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD5 was performed by loading 25 µg of CEM cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD5 monoclonal antibody (Product # MA5-13308) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~67 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CD5 Monoclonal Antibody (4C7) (Product # MA5-13308) and a 54 kDa band corresponding to T-cell surface glycoprotein CD5 was observed in Jurkat and MOLT-4 (T-cells) but not in Raji and Ramos (B-cells) which are reported to be negative for CD5 expression. Membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), MOLT-4 (Lane 2), Raji (Lane 3) and Ramos (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:100 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL1000 (Product # A32752). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with CD5 antibody using peroxidase-conjugate and AEC chromogen. Note intense cell membrane staining of T cells.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD5 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD5 monoclonal antibody (Product # MA5-13308) at a dilution of 1:2 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Immunohistochemistry analysis of each marker in DLBCL cells. A-G) DLBCL cells were positive for CD5 (A), CD10 (B), BCL6 (C), MUM1 (D), BCL2 (E), Ki-67 (F), and C-MYC (G). H) All cases showed diffuse positive staining for CD20. I) Most cases showed diffuse positive for PAX5, but some cases showed heterogeneous and/or weak PAX5 expression.