Antibody data
- Antibody Data
- Antigen structure
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- Product number
- NB100-64227 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-64227, RRID:AB_964546
- Product name
- Mouse Monoclonal MHC Class II RT1Bu Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. NB100-65011 recognizes a polymorphic determinant of rat Ia antigen (RT1Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). This antibody is useful for distinguishing Ia positive cells from different rat strains, e.g. for recognizing cells of donor origin in bone marrow reconstituted radiation chimaeras (3,4). MRC OX-3 cross reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. NB100-65011 recognizes Ia antigens on B-cells, dendritic cells and certain epithelial cells (1,3,4). This product is routinely tested in flow cytometry on Lewis rat splenocytes.
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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