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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-61658 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Neutrophil Elastase/ELA2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against human ELANE (AA: 140-267) recombinant protein. (Expected MW is 39 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mouse mAb against U937 (1), SPC-A-1 (2), and COS7 (3) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis of paraffin-embedded stomach cancer tissues using ELANE mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis of Hela cells using ELANE mouse mAb (green) and negative control (red).
