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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-55553 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-55553, RRID:AB_831049
- Product name
- Anti-CSNK1D
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references High-throughput chemical screen identifies a novel potent modulator of cellular circadian rhythms and reveals CKIα as a clock regulatory kinase.
Hirota T, Lee JW, Lewis WG, Zhang EE, Breton G, Liu X, Garcia M, Peters EC, Etchegaray JP, Traver D, Schultz PG, Kay SA
PLoS biology 2010 Dec 14;8(12):e1000559
PLoS biology 2010 Dec 14;8(12):e1000559
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 229, 107, 78, 46, 31, 26, 16.2
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 47
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
