GTX24775

antibody from GeneTex

Targeting: EIF2B5 EIF-2B, EIF2Bepsilon

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX24775

Provider

GeneTex

Proper citation

GeneTex Cat#GTX24775, RRID:AB_380420

Product name

EIF2B epsilon (phospho Ser539) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Muscle ectopic fat deposition contributes to anabolic resistance in obese sarcopenic old rats through eIF2α activation.

Tardif N, Salles J, Guillet C, Tordjman J, Reggio S, Landrier JF, Giraudet C, Patrac V, Bertrand-Michel J, Migne C, Collin ML, Chardigny JM, Boirie Y, Walrand S
Aging cell 2014 Dec;13(6):1001-11

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Enhanced rates of muscle protein synthesis and elevated mTOR signalling following endurance exercise in human subjects.

Mascher H, Ekblom B, Rooyackers O, Blomstrand E
Acta physiologica (Oxford, England) 2011 Jun;202(2):175-84

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Blood flow restriction exercise stimulates mTORC1 signaling and muscle protein synthesis in older men.

Fry CS, Glynn EL, Drummond MJ, Timmerman KL, Fujita S, Abe T, Dhanani S, Volpi E, Rasmussen BB
Journal of applied physiology (Bethesda, Md. : 1985) 2010 May;108(5):1199-209

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Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men.

Burd NA, Holwerda AM, Selby KC, West DW, Staples AW, Cain NE, Cashaback JG, Potvin JR, Baker SK, Phillips SM
The Journal of physiology 2010 Aug 15;588(Pt 16):3119-30

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Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men.

Moore DR, Robinson MJ, Fry JL, Tang JE, Glover EI, Wilkinson SB, Prior T, Tarnopolsky MA, Phillips SM
The American journal of clinical nutrition 2009 Jan;89(1):161-8

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Rapamycin administration in humans blocks the contraction-induced increase in skeletal muscle protein synthesis.

Drummond MJ, Fry CS, Glynn EL, Dreyer HC, Dhanani S, Timmerman KL, Volpi E, Rasmussen BB
The Journal of physiology 2009 Apr 1;587(Pt 7):1535-46

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Resistance exercise decreases eIF2Bepsilon phosphorylation and potentiates the feeding-induced stimulation of p70S6K1 and rpS6 in young men.

Glover EI, Oates BR, Tang JE, Moore DR, Tarnopolsky MA, Phillips SM
American journal of physiology. Regulatory, integrative and comparative physiology 2008 Aug;295(2):R604-10

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Recombinant EIF2Bepsilon treated with lambda phosphatase (1) or GSK-3beta (2-5) were added to background extracts and resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4¢XC, then incubated with the EIF2Bepsilon [pS539] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and signals were detected. The data show that only the peptide corresponding to EIF2Bepsilon [pS539] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific