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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- MA5-38175 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF2b epsilon Recombinant Rabbit Monoclonal Antibody (ARC1774)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: HeLa, HT-29, U-87MG
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- ARC1774
- Vial size
- 100 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF2b epsilon using eIF2b epsilon Recombinant Monoclonal Antibody (Product # MA5-38175) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of eIF2b epsilon in NIH-3T3 cells using eIF2b epsilon Recombinant Monoclonal Antibody (Product # MA5-38175) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of eIF2b epsilon in U-2 OS cells using eIF2b epsilon Recombinant Monoclonal Antibody (Product # MA5-38175) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of eIF2b epsilon in rat brain tissue using eIF2b epsilon Recombinant Monoclonal Antibody (Product # MA5-38175) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of eIF2b epsilon in human lung cancer tissue using eIF2b epsilon Recombinant Monoclonal Antibody (Product # MA5-38175) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of eIF2b epsilon in mouse kidney tissue using eIF2b epsilon Recombinant Monoclonal Antibody (Product # MA5-38175) at a dilution of 1:100.
