13791-1-AP
antibody from Invitrogen Antibodies
Targeting: SPART
KIAA0610, SPG20, TAHCCP1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [2]
- Other assay [1]
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Validation data
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- Product number
- 13791-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Spartin/SPG20 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEQEPQNGE PAEIKIIREA YKKAFLFVNK GLNTDELGQK EEAKNYYKQG IGHLLRGISI SSKESEHTGT GWESARQMQQ KMKETLQNVR TRLEILEKGL ATSLQNDLQE VPKLYPEFPP KDMCEKLPEP QSFSSAPQHA EVNGNTSTPS AGAVAAPASL SLPSQSCPAE APPAYTPQAA EGHYTVSYGT DSGEFSSVGE EFYRNHSQPP PLETLGLDAD ELILIPNGVQ IFFVNPAGEV SAPSYPGYLR IVRFLDNSLD TVLNRPPGFL QVCDWLYPLV PDRSPVLKCT AGAYMFPDTM LQAAGCFVGV VLSSELPEDD RELFEDLLRQ MSDLRLQANW NRAEEENEFQ I (1-350 aa encoded by BC047083)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.15 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 13791-1-AP (Spartin, SPG20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using SPG20 antibody 13791-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13791-1-AP (Spartin, SPG20 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13791-1-AP (Spartin, SPG20 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas using 13791-1-AP (Spartin, SPG20 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were intracellularly stained with 0.2 µg Anti-Human Spartin, SPG20 (Product # 13791-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug Spartin, SPG20 antibody (13791-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Spartin, SPG20 (IP:13791-1-AP, 3ug; Detection:13791-1-AP 1:1000) with mouse brain tissue lysate 6000ug.