PA5-83748

antibody from Invitrogen Antibodies

Targeting: PIK3CD p110D

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-83748

Provider

Invitrogen Antibodies

Product name

PIK3CD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AECSRLLQIL ELGRHSECVH VTEEEQLQLR EILERRGSGE LYEHEKDLVW KLRHEVQEHF PEALARLLLV TKWN

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs.

Zhang L, Li X, Shi C, Ran G, Peng Y, Zeng S, He Y
Stem cells international 2021;2021:5594370

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Hyoscyamus albus nortropane alkaloids reduce hyperglycemia and hyperinsulinemia induced in HepG2 cells through the regulation of SIRT1/NF-kB/JNK pathway.

Kowalczuk A, Bourebaba N, Kornicka-Garbowska K, Turlej E, Marycz K, Bourebaba L
Cell communication and signaling : CCS 2021 May 25;19(1):61

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIK3CD by a PIK3CD polyclonal antibody (Product # PA5-83748). Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human Tonsil tissue.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PIK3CD in U-2 OS cells using a PIK3CD polyclonal antibody (Product # PA5-83748). The analysis shows localization to cytokinetic bridge & vesicles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PIK3CD in human lymph node and colon tissues using a PIK3CD polyclonal antibody (Product # PA5-83748). Corresponding RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PIK3CD in human lymph node using a PIK3CD polyclonal antibody (Product # PA5-83748). The analysis shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PIK3CD in human colon using a PIK3CD polyclonal antibody (Product # PA5-83748). The analysis shows low expression as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 Effect of total calystegines on collapsed insulin signalling pathway in HI/HG HepG2 cells. a Quantitative analysis of insulin signalling-related proteins expression using western blot. b Representative blots images of insulin signalling-related proteins profiling. c Relative genes expression of key insulin signalling regulators. Representative data from three independent experiments are shown +- SD (n = 3). An asterisk (*) indicates a comparison of IR group to untreated healthy cells. A hashtag (#) indicates a comparison of IR group pre-treated with calystegines to IR untreated healthy cells. */# p < 0.05, **/## p < 0.01, ***/### p < 0.001. HepG2_HE: HepG2 healthy untreated cells; HepG2_IR: Insulin resistant HepG2 cells exposed to high concentrations of insulin and glucose. HepG2_IR- H. albus _Caly: Insulin resistant HepG2 cells exposed to high concentrations of insulin and glucose and pre-treated with 250 mug/ml calystegines extracted from Hyoscyamus albus seeds

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 (a) qRT-PCR analysis of the CD34, VEGF, MMP9, and SDF-1 expressions in each group. (b) Western blot analysis results of CD34, VEGF, MMP9, and SDF-1 protein expressions in each group. * P < 0.05 compared with the blank group.