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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF8199 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GM130/GOLGA2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human GM130/GOLGA2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q08379- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The potassium channel KCNJ13 is essential for smooth muscle cytoskeletal organization during mouse tracheal tubulogenesis.
Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells.
CLIPR-59: a protein essential for neuromuscular junction stability during mouse late embryonic development.
Yin W, Kim HT, Wang S, Gunawan F, Wang L, Kishimoto K, Zhong H, Roman D, Preussner J, Guenther S, Graef V, Buettner C, Grohmann B, Looso M, Morimoto M, Mardon G, Offermanns S, Stainier DYR
Nature communications 2018 Jul 19;9(1):2815
Nature communications 2018 Jul 19;9(1):2815
Endosomal phosphatidylserine is critical for the YAP signalling pathway in proliferating cells.
Matsudaira T, Mukai K, Noguchi T, Hasegawa J, Hatta T, Iemura SI, Natsume T, Miyamura N, Nishina H, Nakayama J, Semba K, Tomita T, Murata S, Arai H, Taguchi T
Nature communications 2017 Nov 1;8(1):1246
Nature communications 2017 Nov 1;8(1):1246
CLIPR-59: a protein essential for neuromuscular junction stability during mouse late embryonic development.
Couesnon A, Offner N, Bernard V, Chaverot N, Backer S, Dimitrov A, Perez F, Molgó J, Bloch-Gallego E
Development (Cambridge, England) 2013 Apr;140(7):1583-93
Development (Cambridge, England) 2013 Apr;140(7):1583-93
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human GM130/GOLGA2 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and ZR-75 human breast cancer cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GM130/GOLGA2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GM130/GOLGA2 in HeLa Human Cell Line. GM130/GOLGA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human GM130/GOLGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8199) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to Golgi apparatus vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
