LS-C676017

antibody from LSBio

Targeting: MAGOH MAGOH1, MAGOHA

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C676017

Provider

LSBio

Product name

MAGOH Antibody LS-C676017

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All Lanes: MAGOH antibody at 4.36 ug/ml Lane 1: Mouse kidney tissue Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: K562 whole cell lysate Lane 6: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 18,13 kDa Observed band size: 17 kDa

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: MAGOH antibody at 4.36µg/ml Lane 1: Mouse kidney tissue Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: K562 whole cell lysate Lane 6: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 18, 13 kDa Observed band size: 18 kDa

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All Lanes: MAGOH antibody at 4.36 ug/ml Lane 1: Mouse kidney tissue Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: K562 whole cell lysate Lane 6: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 18,13 kDa Observed band size: 17 kDa

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot All lanes: MAGOH antibody at 4.36µg/ml Lane 1: Mouse kidney tissue Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Raji whole cell lysate Lane 5: K562 whole cell lysate Lane 6: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 18, 13 kDa Observed band size: 18 kDa

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitating MAGOH in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating MAGOH in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitating MAGOH in HeLa whole cell lysate Lane 1: Rabbit monoclonal IgG(1ug)instead of product in HeLa whole cell lysate.For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: product(4ug)+ HeLa whole cell lysate(500ug) Lane 3: HeLa whole cell lysate (20ug)