PA5-118265

antibody from Invitrogen Antibodies

Targeting: SLC25A15 D13S327, HHH, ORC1, ORNT1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118265

Provider

Invitrogen Antibodies

Product name

ORNT1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ORNT1 Polyclonal Antibody (Product # PA5-118265) at 1:500 dilution. Lane A: Raji Whole Cell Lysate, Lane B: Mouse liver tissue lysate Whole Cell Lysate, Lane C: U-251MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa. (We are unsure of the identity of these extra bands).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ORNT1 Polyclonal Antibody (Product # PA5-118265) at 1:500 dilution. Lane A: Raji Whole Cell Lysate, Lane B: Mouse liver tissue lysate Whole Cell Lysate, Lane C: U-251MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa. (We are unsure of the identity of these extra bands).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ORNT1 in human kidney with ORNT1 Polyclonal Antibody (Product # PA5-118265, 1:300 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ORNT1 in human liver with ORNT1 Polyclonal Antibody (Product # PA5-118265, 1:300 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ORNT1 Immunoprecipitation using: Lane A: 0.5 mg mouse liver Whole Cell Lysate 4 µL with ORNT1 Polyclonal Antibody (Product # PA5-118265) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: ORNT1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa.