A305-693A-T

antibody from Invitrogen Antibodies

Targeting: WBP4 FBP21, MGC117310

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A305-693A-T

Provider

Invitrogen Antibodies

Product name

WBP4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human WBP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WBP4 antibody A305-693A-M (lot A305-693A-M-1) used for IP at 8 µl per reaction. WBP4 was also immunoprecipitated by rabbit anti-WBP4 antibody BL22618. For blotting immunoprecipitated WBP4, A305-693A-M was used at 1:400. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human WBP4 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WBP4 antibody A305-693A-M (lot A305-693A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.