12361-1-AP

antibody from Invitrogen Antibodies

Targeting: HBE1 HBE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

12361-1-AP

Provider

Invitrogen Antibodies

Product name

Hemoglobin epsilon Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis.

Concentration

0.39 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12361-1-AP (Hemoglobin epsilon antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human placenta using 12361-1-AP (Hemoglobin epsilon antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 K-562 cells were stained with 0.2ug Hemoglobin epsilon antibody (12361-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.