Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-24277 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FBXO15 Monoclonal Antibody (612834)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 612834
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of FBXO15 in immersion fixed BG01V human embryonic stem cells. Samples were incubated in FBXO15 monoclonal antibody (Product # MA5-24277) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green). Specific staining of F‚box protein 15 was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of FBXO15 in BG01V human embryonic stem cells. Samples were incubated in FBXO15 monoclonal antibody (Product # MA5-24277) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.