Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB6035 - Provider product page
- Provider
- R&D Systems
- Product name
- Human F-box protein 15/FBXO15 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human F-box protein 15/FBXO15 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse F-box protein 15 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q8NCQ5-2- Isotype
- IgG
- Antibody clone number
- 612834
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- F-box protein 15/FBXO15 in BG01V Human Embryonic Stem Cells. F-box protein 15/FBXO15 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human F-box protein 15/FBXO15 Monoclonal Antibody (Catalog # MAB6035) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Oct-3/4 was detected in cell nuclei using Goat Anti-Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759) and stained using NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Specific staining of F-box protein 15 was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of FBXO15 in BG01V Human Stem Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human F-box protein 15/FBXO15 Monoclonal Antibody (Catalog # MAB6035, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
