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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- A300-223A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cul7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse Cul7 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, HEK293T, HeLa, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul7 antibody (Product # A300-223A; lot A300-223A-4) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Cul7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cul7 antibody (Product # A300-223A; lot A300-223A-4) used for IP at 6 µg per reaction. Cul7 was also immunoprecipitated by a previous lot of this antibody (lot A300-223A-3) and rabbit anti-Cul7 antibody (Product # A300-224A). For blotting immunoprecipitated Cul7, Product # A300-223A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
