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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
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Validation data
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- Product number
- 17826-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPERT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MQPEGLKCR MEESNAERGT AEPFPRLHNL YSTPRCAQQA ALPRLSRRMA SQHSYPLNRF SSVPLDPMER PMSQADLELD YNPPRVQLSD EMFVFQDGRW VNENCRLQSP YFSPSASFHH KLHHKRLAKE CMLQEENKSL REENKALREE NRMLSKENKI LQVFWEEHKA SLGREESRAP SPLLHKDSAS LEVVKKDHVA LQVPRGKEDS TLQLLREENR ALQQLLEQKQ AYWAQAEDTA APAEESKPAP SPHEEPCSPG LLQDQGSGLS SRFEEPKGPP ARQEDSKELR ALRKMVSNMS GPSGEEEAKV GPGLPDGCQP LQLLREMRQA LQALLKENRL LQEENRTLQV LRAEHRGFQE ENKALWENNK LKLQQKLVID TVTEVTARME MLIEELYAFM PARSQDPKKP SRV (1-412 aa encoded by BC027609)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 17826-1-AP (SPERT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SPERT antibody (Product # 17826-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using SPERT antibody 17826-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
