LS-B14865

antibody from LSBio

Targeting: KPNA2 IPOA1, QIP2, RCH1, SRP1alpha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-B14865

Provider

LSBio

Product name

IHC-plus™ KPNA2 / Importin Alpha 1 Antibody LS-B14865

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using KPNA2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of U-251MG cells, using 3 ug KPNA2 antibody. Western blot was performed from the immunoprecipitate using KPNA2 antibodyat a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of U-251MG cells, using 3 ug KPNA2 antibody. Western blot was performed from the immunoprecipitate using KPNA2 antibodyat a dilition of 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation analysis of 200ug extracts of U-251MG cells, using 3 ug KPNA2 antibody. Western blot was performed from the immunoprecipitate using KPNA2 antibodyat a dilition of 1:1000.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using KPNA2 antibody at dilution of 1:200 (400x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung using KPNA2 antibody at dilution of 1:200 (200x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human stomach cancer using KPNA2 antibody at dilution of 1:200 (400x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat brain using KPNA2 antibody at dilution of 1:200 (200x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney using KPNA2 antibody at dilution of 1:200 (400x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung using KPNA2 antibody at dilution of 1:200 (200x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human stomach cancer using KPNA2 antibody at dilution of 1:200 (400x lens).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat brain using KPNA2 antibody at dilution of 1:200 (200x lens).