MA5-31790

antibody from Invitrogen Antibodies

Targeting: KPNA2 IPOA1, QIP2, RCH1, SRP1alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31790

Provider

Invitrogen Antibodies

Product name

KPNA2 Monoclonal Antibody (1E8B7)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

1E8B7

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KPNA2 in HeLa (1), HEK293 (2), and NIH/3T3 (3) cell lysate. Samples were incubated with KPNA2 monoclonal antibody (Product # MA5-31790) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KPNA2 in human KPNA2 recombinant protein. Sample was incubated with KPNA2 monoclonal antibody (Product # MA5-31790) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KPNA2 in HEK293 (1) and KPNA2 cell lysate. Samples were incubated with KPNA2 monoclonal antibody (Product # MA5-31790) using a dilution of 1:500-1:2000.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of KPNA2 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with KPNA2 monoclonal antibody (Product # MA5-31790) using a dilution of 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of KPNA2 in Hela cells (green). Sample was incubated with KPNA2 monoclonal antibody (Product # MA5-31790) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KPNA2 in paraffin-embedded lung cancer tissue. Sample was incubated with KPNA2 monoclonal antibody (Product # MA5-31790) using a dilution of 1:200-1:1000 followed by DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KPNA2 in paraffin-embedded esophageal cancer tissue. Sample was incubated with KPNA2 monoclonal antibody (Product # MA5-31790) using a dilution of 1:200-1:1000 followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of KPNA2 in HeLa cells (green). Sample was incubated with KPNA2 monoclonal antibody (Product # MA5-31790) using a dilution of 1:200-1:400 followed by negative control (red).