Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- 600-401-C69 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-C69, RRID:AB_2613248
- Product name
- Anti-IDO2 (RABBIT) Antibody - 600-401-C69
- Antibody type
- Polyclonal
- Description
- Anti-IDO2 antibody is directed against IDO2 protein. The rabbit polyclonal antibody was affinity purified against the antigen. Prior to affinity purification the antibody was pre-cleared of any cross reactive antibody to IDO1 (human and mouse). A BLAST analysis was used to suggest cross-reactivity with IDO2 protein from human and mouse 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store vial at -20°C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Handling
- Dry Ice
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Immunofluorescence of Anti-IDO2 Antibody. Tissue: human IDO2 expressing HEK293 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary: IDO2 antibody at 1:50-1:1000 for 1 h at RT. Secondary: Peroxidase rabbit secondary antibody at 1:10,000 for 45min at RT. Localization: IDO-2 is located in the cytosol. Staining: IDO-2 as green fluorescent signal with bis-benzimide nuclear counterstain (blue).
- Sample type
- IF Microscopy