600-401-C69

antibody from Rockland Immunochemicals, Inc.

Targeting: IDO2 INDOL1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

600-401-C69

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-C69, RRID:AB_2613248

Product name

Anti-IDO2 (RABBIT) Antibody - 600-401-C69

Antibody type

Polyclonal

Description

Anti-IDO2 antibody is directed against IDO2 protein. The rabbit polyclonal antibody was affinity purified against the antigen. Prior to affinity purification the antibody was pre-cleared of any cross reactive antibody to IDO1 (human and mouse). A BLAST analysis was used to suggest cross-reactivity with IDO2 protein from human and mouse 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Handling

Dry Ice

Immunocytochemistry

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Immunofluorescence of Anti-IDO2 Antibody. Tissue: human IDO2 expressing HEK293 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary: IDO2 antibody at 1:50-1:1000 for 1 h at RT. Secondary: Peroxidase rabbit secondary antibody at 1:10,000 for 45min at RT. Localization: IDO-2 is located in the cytosol. Staining: IDO-2 as green fluorescent signal with bis-benzimide nuclear counterstain (blue).

Sample type

IF Microscopy