Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- PA1612 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-INDOL1/IDO2 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for IDO2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, FCM. Reactive species: Human. IDO2 information: Molecular Weight: 45424 MW; Tissue Specificity: Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Flow Cytometry analysis of SiHa cells using anti-IDO2 antibody (PA1612). Overlay histogram showing SiHa cells stained with PA1612 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDO2 Antibody (PA1612, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
- Additional image