PA1612

antibody from Boster Biological Technology

Targeting: IDO2 INDOL1

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1612

Provider

Boster Biological Technology

Product name

Anti-INDOL1/IDO2 Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for IDO2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, FCM. Reactive species: Human. IDO2 information: Molecular Weight: 45424 MW; Tissue Specificity: Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of SiHa cells using anti-IDO2 antibody (PA1612). Overlay histogram showing SiHa cells stained with PA1612 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDO2 Antibody (PA1612, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image