AMAb91440

antibody from Atlas Antibodies

Targeting: CD47 IAP, MER6, OA3

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb91440

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91440, RRID:AB_2732097

Product name

Anti-CD47

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

AQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTT
EVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIE
VSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTRE
GETIIELKYRVVS


Epitope

Binds to an epitope located within the peptide sequence SAKIEVSQLL as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL6913

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using AMAb91440 antibody. Corresponding CD47 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lung shows moderate membranous positivity in pneumocytes.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows strong membranous positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.