MA5-32694

antibody from Invitrogen Antibodies

Targeting: ABCC1 GS-X, MRP, MRP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32694

Provider

Invitrogen Antibodies

Product name

MRP1 Recombinant Rabbit Monoclonal Antibody (JA93-03)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JA93-03

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-MRP1 Recombinant Rabbit Monoclonal Antibody (JA93-03) (Product # MA5-32694) and a 150-220 kDa band corresponding to ABCC1 was observed across cell lines tested. Membrane enriched extracts (40 µg lysate) of A549 (Lane 1), HeLa (Lane 2), Hep G2 (Lane 3), BeWo (Lane 4) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ABCC1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MRP1 Recombinant Rabbit Monoclonal Antibody (JA93-03) (Product # MA5-32694) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane, cell junction and nuclear localization. Panel e represents the negative expression model, BeWo. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MRP1 of paraffin-embedded Human pancreas tissue using a MRP1 Monoclonal antibody (Product #MA5-32694).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MRP1 of paraffin-embedded Human tonsil tissue using a MRP1 Monoclonal antibody (Product #MA5-32694). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of MRP1 in Hela cells using a MRP1 Monoclonal Antibody (Product # MA5-32694) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of MRP1 in Hela cells using a MRP1 Monoclonal Antibody (Product # MA5-32694) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).