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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-30594 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431.
- Concentration
- 1.73 mg/mL
Submitted references Metformin inhibits proliferation and enhances chemosensitivity of intrahepatic cholangiocarcinoma cell lines.
Metformin reverses multidrug resistance in human hepatocellular carcinoma Bel‑7402/5‑fluorouracil cells.
Ling S, Feng T, Ke Q, Fan N, Li L, Li Z, Dong C, Wang C, Xu F, Li Y, Wang L
Oncology reports 2014 Jun;31(6):2611-8
Oncology reports 2014 Jun;31(6):2611-8
Metformin reverses multidrug resistance in human hepatocellular carcinoma Bel‑7402/5‑fluorouracil cells.
Ling S, Tian Y, Zhang H, Jia K, Feng T, Sun D, Gao Z, Xu F, Hou Z, Li Y, Wang L
Molecular medicine reports 2014 Dec;10(6):2891-7
Molecular medicine reports 2014 Dec;10(6):2891-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRP1 using 30 µg of A431 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a MRP1 polyclonal antibody (Product # PA5-30594) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MRP1 Polyclonal Antibody (Product # PA5-30594). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with MRP1 Polyclonal Antibody (Product # PA5-30594) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using MRP1 (Product # PA5-30594) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Met affects the expression of HIF-1alpha, MDR1/P-gp and MRP1. (A) Reverse transcription-polymerase chain reaction analysis of MDR1 and MRP1 expression in the different treatment groups. (B) Western blot analysis of HIF-1alpha, MDR1 and MRP1 in the different treatment groups. (C-E) Relative density of the western blotting bands demonstrated that levels of HIF-1alpha, P-gp and MRP1 decreased with increasing concentrations of Met in a dose-dependent manner, with a statistically significant difference between the treatments and the controls ( ** P
