Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX101512 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101512, RRID:AB_10731496
- Product name
- CD19 antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Kaposi's sarcoma-associated herpesvirus-positive primary effusion lymphoma tumor formation in NOD/SCID mice is inhibited by neomycin and neamine blocking angiogenin's nuclear translocation.
Bottero V, Sadagopan S, Johnson KE, Dutta S, Veettil MV, Chandran B
Journal of virology 2013 Nov;87(21):11806-20
Journal of virology 2013 Nov;87(21):11806-20
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CD19 antibody detects CD19 protein by western blot analysis.A. 30 £gg Raji whole cell lysate/extract7.5 % SDS-PAGECD19 antibody (GTX101512) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] (GTX101512) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CD19 antibody [C1C3] detects CD19 protein at membrane by immunofluorescent analysis.Sample: Raji cells were fixed in ice-cold MeOH for 5 min.Green: CD19 protein stained by CD19 antibody [C1C3] (GTX101512) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CD19 antibody [C1C3] (GTX101512) detects CD19 protein by flow cytometry analysis. Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4?C for 15 min. Black: Unlabelled sample was used as a control. Red: CD19 antibody [C1C3] (GTX101512) dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
