20778-1-AP

antibody from Invitrogen Antibodies

Targeting: WDR24 C16orf21, DKFZp434F054, JFP7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

20778-1-AP

Provider

Invitrogen Antibodies

Product name

WDR24 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LGRNQVAQT WTMLRIIYCS PGLVPTANLN HSVGKGGSCG LPLMNSFNLK DMAPGLGSET RLDRSKGDAR SDTVLLDSSA TLITNEDNEE TEGSDVPADY LLGDVEGEED ELYLLDPEHA HPEDPECVLP QEAFPLRHEI VDTPPGPEHL QDKADSPHVS GSEADVASLA PVDSSFSLLS VSHALYDSRL PPDFFGVLVR DMLHFYAEQG DVQMAVSVLI VLGERVRKDI DEQTQEHWYT SYIDLLQRFR LWNVSNEVVK LSTSRAVSCL NQASTTLHVN CSHCKRPMSS RGWVCDRCHR CASMCAVCHH VVKGLFVWCQ GCSHGGHLQH IMKWLEGSSH CPAGCGHLCE YS (570-920 aa encoded by BC008025)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.25 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon using 20778-1-AP (WDR24 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon using 20778-1-AP (WDR24 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-WDR24 (IP:20778-1-AP, 4ug; Detection:20778-1-AP 1:500) with HeLa cells lysate 3200ug.