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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- NBP2-16008 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal CREB3L3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Potential contribution of ryanodine receptor 2 upregulation to cGMP/PKG signaling-induced cone degeneration in cyclic nucleotide-gated channel deficiency.
Yang F, Ma H, Butler MR, Ding XQ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6335-6350
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 May;34(5):6335-6350
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CREB3L3 Antibody [NBP2-16008] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CREB3L3 Antibody [NBP2-16008] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB3L3 antibody [N1C1] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CREB3L3 Antibody [NBP2-16008] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CREB3L3 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CREB3L3 Antibody [NBP2-16008] - U251 xenograft, using CREB3L3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CREB3L3 Antibody [NBP2-16008] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CREB3L3 stained by CREB3L3 antibody [N1C1] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.
