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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-31708 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CREB3L3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.81 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CREB3L3 using 30 µg of IMR32 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a CREB3L3 polyclonal antibody (Product # PA5-31708) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CREB3L3 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CREB3L3 Polyclonal Antibody (Product # PA5-31708) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CREB3L3 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CREB3L3 Polyclonal Antibody (Product # PA5-31708) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of CREB3L3 was performed in A431 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CREB3L3 Polyclonal Antibody (Product # PA5-31708) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
