H00005413-D01

antibody from Abnova Corporation

Targeting: SEPTIN5 H5, HCDCREL-1, PNUTL1, SEPT5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005413-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005413-D01, RRID:AB_10721360

Product name

SEPT5 MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human SEPT5 protein.

Antigen sequence

MSTGLRYKSKLATPEDKQDIDKQYVGFATLPNQVH
RKSVKKGFDFTLMVAGESGLGKSTLVHSLFLTDLY
KDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLT
IVDTPGFGDAVNNTECWKPITDYVDQQFEQYFRDE
SGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFM
KALHEKVNIVPLIAKADCLVPSEIRKLKERIREEI
DKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFA
VIGSNTVVEAKGQRVRGRLYPWGIVEVENQAHCDF
VKLRNMLIRTHMHDLKDVTCDVHYENYRAHCIQQM
TSKLTQDSRMESPIPILPLPTPDAETEKLIRMKDE
ELRRMQEMLQRMKQQMQDQ


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SEPT5 MaxPab rabbit polyclonal antibody. Western Blot analysis of SEPT5 expression in HeLa.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SEPT5 expression in transfected 293T cell line (H00005413-T04) by SEPT5 MaxPab polyclonal antibody.Lane 1: SEPT5 transfected lysate(42.8 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to SEPT5 on HeLa cell. [antibody concentration 20 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of SEPT5 transfected lysate using anti-SEPT5 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SEPT5 purified MaxPab mouse polyclonal antibody (B02P) (H00005413-B02P).

Validation comment

Immunoprecipitation

Protocol

Protocol