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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 11631-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEPT5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSTGLRYKS KLATPEDKQD IDKQYVGFAT LPNQVHRKSV KKGFDFTLMV AGESGLGKST LVHSLFLTDL YKDRKLLSAE ERISQTVEIL KHTVDIEEKG VKLKLTIVDT PGFGDAVNNT ECWKPITDYV DQQFEQYFRD ESGLNRKNIQ DNRVHCCLYF ISPFGHGLRP VDVGFMKALH EKVNIVPLIA KADCLVPSEI RKLKERIREE IDKFGIHVYQ FPECDSDEDE DFKQQDRELK ESAPFAVIGS NTVVEAKGQR VRGRLYPWGI VEVENQAHCD FVKLRNMLIR THMHDLKDVT CDVHYENYRA HCIQQMTSKL TQDSRMESPI PILPLPTPDA ETEKLIRMKD EELRRMQEML QRMKQQMQDQ (1-369 aa encoded by BC025261)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 11631-1-AP (SEPT5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 11631-1-AP (SEPT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 11631-1-AP (SEPT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11631-1-AP (SEPT5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11631-1-AP ( SEPT5 antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 11631-1-AP ( SEPT5 antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SEPT5 (IP:11631-1-AP, 3ug; Detection:11631-1-AP 1:300) with mouse brain tissue lysate 1000ug.
