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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- DM1219 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DM1219, RRID:AB_10655709
- Product name
- anti UBXN6
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human UBXD1 purified from bacteria.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5C3-1
- Vial size
- 50 µg
- Concentration
- 0.1 mg/ml
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Legend: U2OS were co-transfected with pBabe-puro and pSuper based plasmid encoding anti-UBXD1 shRNA (+) or mismatch control (-). Cells were selected in puromycin for 3 days. After selection, cells were harvested and protein extracts prepared. Proteins were resolved by SDS-PAGE, blotted onto nitrocellulose membrane and probed with the indicated antibodies. A picture of the Ponceau S stained blot after processing is shown to demonstrate equivalent loading of protein.
