A700-042
antibody from Invitrogen Antibodies
Targeting: TNFRSF4
ACT35, CD134, OX40, TXGP1L
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- A700-042 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OX40/CD134 Recombinant Rabbit Monoclonal Antibody (BLR042F)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR042F
- Vial size
- 100 µL
- Concentration
- 50 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human OX40/CD134 by western blot. Samples: Whole cell lysate (50 µg) from HH, HeLa, MJ, HEK293T, Hep-G2, C5/MJ, Jurkat, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody [BLR042F] (Product # A700-042 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower panel: Rabbit anti-eEF2 (Product # A301-688A).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human OX40/CD134 by immunocytochemistry. Sample: FFPE section of MJ cells. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody [BLR042F] (Product # A700-042 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human OX40/CD134 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody [BLR042F] (Product # A700-042 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human OX40/CD134 by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody (BLR042F) (A700-042 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human OX40/CD134 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal antibody [BLR042F] (Product # A700-042 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human OX40/CD134 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-OX40/CD134 recombinant monoclonal [BLR042F] (Product #A700-042; lot 1) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human OX40/CD134 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-OX40/CD134 recombinant monoclonal antibody [BLR042F] (Product # A700-042 lot 1) used for IP at 20 µL per reaction. OX40/CD134 was also immunoprecipitated by rabbit anti-OX40/CD134 recombinant monoclonal antibody [548-1D12]. For blotting immunoprecipitated OX40/CD134 (Product # A700-042) was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.