Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- sc-21756 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-21756, RRID:AB_2295004
- Product name
- Anti-HBG2
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Systematic documentation and analysis of human genetic variation in hemoglobinopathies using the microattribution approach.
Productive Parvovirus B19 Infection of Primary Human Erythroid Progenitor Cells at Hypoxia Is Regulated by STAT5A and MEK Signaling but not HIFα.
Immunoblotting conditions for human hemoglobin chains.
Giardine B, Borg J, Higgs DR, Peterson KR, Philipsen S, Maglott D, Singleton BK, Anstee DJ, Basak AN, Clark B, Costa FC, Faustino P, Fedosyuk H, Felice AE, Francina A, Galanello R, Gallivan MV, Georgitsi M, Gibbons RJ, Giordano PC, Harteveld CL, Hoyer JD, Jarvis M, Joly P, Kanavakis E, Kollia P, Menzel S, Miller W, Moradkhani K, Old J, Papachatzopoulou A, Papadakis MN, Papadopoulos P, Pavlovic S, Perseu L, Radmilovic M, Riemer C, Satta S, Schrijver I, Stojiljkovic M, Thein SL, Traeger-Synodinos J, Tully R, Wada T, Waye JS, Wiemann C, Zukic B, Chui DH, Wajcman H, Hardison RC, Patrinos GP
Nature genetics 2011 Mar 20;43(4):295-301
Nature genetics 2011 Mar 20;43(4):295-301
Productive Parvovirus B19 Infection of Primary Human Erythroid Progenitor Cells at Hypoxia Is Regulated by STAT5A and MEK Signaling but not HIFα.
Chen AY, Kleiboeker S, Qiu J
PLoS pathogens 2011 Jun;7(6):e1002088
PLoS pathogens 2011 Jun;7(6):e1002088
Immunoblotting conditions for human hemoglobin chains.
Suzuki Y, Takeda Y, Ikuta T
Analytical biochemistry 2008 Jul 15;378(2):218-20
Analytical biochemistry 2008 Jul 15;378(2):218-20
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 17
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human spleen shows weak cytoplasmic positivity in a subset of cells in red pulp.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
