HPA019000

antibody from Atlas Antibodies

Targeting: PHLDA1 DT1P1B11, PHRIP, TDAG51

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA019000

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA019000, RRID:AB_1855309

Product name

Anti-PHLDA1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

RMLESSGCKALKEGVLEKRSDGLLQLWKKKCCILT
EEGLLLIPPKQLQHQQQQQQQQQQQQQQPGQGPAE
PSQPSGPAVASLEPPVKLKELHFSNMKTVDCVE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines SK-MEL-30 and HeLa using Anti-PHLDA1 antibody. Corresponding PHLDA1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human salivary gland shows strong positivity in glandular cells.

Sample type

HUMAN